Product Name: Apaf1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 – 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 132 kDa Observed: 130 kDa
Immunogen: Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1.The sequences of the immunogenic peptide are identical between human and mouse.The immunogen is located within the first 50 amino acids of Apaf1.
Host Species: Rabbit
Purification: Apaf1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 839706-07-9
Product: GNF-7
Buffer: Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
Storage Conditions: Apaf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1
Accession NO.: AAC51678
Protein Ino: 2330015
Official Symbol: APAF1
Geneid: 317
Background: Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
PubMed ID:http://aac.asm.org/content/53/2/651.abstract
