Product Name: Amphiphysin Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.
Host Species: Goat
Purification: Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1243245-18-2
Product: GNF-6231
Buffer: AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AMPH, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen), HGNC:471, AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
Accession NO.: NP_001626.1, NP_647477.1
Protein Ino: 4502081
Official Symbol: AMPH
Geneid: 273
Background:
PubMed ID:http://aac.asm.org/content/52/10/3648.abstract
