Product Name: Adiponectin Antibody [MADI 04]
Species Reactivity: Mouse
Tested Applications: ELISA, IP, WB
Applications: ELISA: (direct and indirect: 1:5,000-1:10,000). Immunoprecipitation: (1-5ul per sample in a final reaction volume of 500-1,000ul). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant mouse adiponectin.
Host Species: Rat
Purification:
Physical State: Liquid
CAS NO.: 223499-30-7
Product: YM-58483
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Accession NO.: Q60994
Protein Ino: 408359957
Official Symbol: Adipoq
Geneid: 11450
Background: ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
PubMed ID:http://aac.asm.org/content/52/5/1589.abstract
