Product Name: Aak1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: Aak1 antibody can be used for detection of Aak1 by Western blot at 1 – 2 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Aak1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human Aak1.The immunogen is located within amino acids 830 – 880 of Aak1.
Host Species: Rabbit
Purification: Aak1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 9007-28-7
Product: Chondroitin (sulfate)
Buffer: Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Aak1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Aak1 Antibody: KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1
Accession NO.: NP_055726
Protein Ino: 148277037
Official Symbol: AAK1
Geneid: 22848
Background: Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
PubMed ID:http://aac.asm.org/content/51/11/3902.abstract
