Product Name: APRIL Antibody [Sacha-1]
Species Reactivity: Human, Mouse
Tested Applications: Flow, ICC
Applications: APRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL), and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5 μg/mL Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APRIL (monoclonal Sache-1) antibody was raised against recombinant human APRIL (aa. 105-250).
Host Species: Rat
Purification: APRIL (monoclonal Sache-1) antibody was purified to ≥95% in SDS-PAGE.
Physical State: Liquid
CAS NO.: 1142090-23-0
Product: GSK2256294A
Buffer: PBS containing 0.02% sodium azide
Concentration:
Storage Conditions: APRIL antibody can be stored at 4˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: APRIL, TALL2, ZTNF2, UNQ383/PRO715, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, TALL-2, TRDL-1
Accession NO.: O75888
Protein Ino: 13124605
Official Symbol: TNFSF13
Geneid: 8741
Background:
PubMed ID:http://aac.asm.org/content/53/3/1185.abstract
