Product Name: APRIL Antibody [Aprily-8]
Species Reactivity: Human
Tested Applications: Flow, ICC, WB
Applications: APRIL antibody can be used to recognize human APRIL by Flow Cytometry, immunocytochemistry and Western Blot. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50˚C. Slides were then successively washed 2x 10min in xylol, 2x 10min in 100% EtOH, and then treated 10 min in 100% MeOH/ 0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% EtOH and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5 μg/mL Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human APRIL. Cells (5×105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10 μg/mL of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 10 μg of protein was applied to the gel, and revealed with Aprily-8 (1 μg/mL) and HRP-coupled anti-mouse secondary antibody (1:4,000).Note: The hAPRIL construct used in this experiment is an uncleavable fusion protein between hBAFF (aa 1-132) and hAPRIL (aa 93-233). This product is for research use only.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APRIL (monoclonal Aprily-8) antibody was raised against recombinant human APRIL (aa. 93-233) expressed in bacteria.
Host Species: Mouse
Purification: APRIL (monoclonal Aprily-8) antibody was purified to >=95% in SDS-PAGE.
Physical State: Liquid
CAS NO.: 1058156-90-3
Product: Anlotinib
Buffer: PBS containing 0.02% sodium azide
Concentration:
Storage Conditions: APRIL antibody can be stored at 4˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: APRIL, TALL2, ZTNF2, UNQ383/PRO715, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, TALL-2, TRDL-1
Accession NO.: O75888
Protein Ino: 13124605
Official Symbol: TNFSF13
Geneid: 8741
Background:
PubMed ID:http://aac.asm.org/content/53/3/912.abstract
