Product Name: APIP Antibody
Species Reactivity: Human
Tested Applications: IHC, WB
Applications: APimmunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APIP antibody was raised against a full – length His – tagged recombinant APIP protein. of APIP (Human).
Host Species: Rabbit
Purification: Protein G Column
Physical State: Liquid
CAS NO.: 465-16-7
Product: Oleandrin
Buffer: PBS, 0.2% gelatin, 0.05% sodium azide.
Concentration:
Storage Conditions: APIP antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APIP, APAF1-interacting protein, APIP2, CGI29, CGI-29, DJ179L10.2, MTRu-1-P dehydratase, APAF1 interacting protein, MMRP19
Accession NO.: Q96GX9
Protein Ino: 74731866
Official Symbol: APIP
Geneid: 51074
Background: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
PubMed ID:http://aac.asm.org/content/53/2/412.abstract
