Product Name: APC7 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, WB
Applications: APC7 antibody can be used for detection of APC7 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 66 kDa Observed: 68 kDa
Immunogen: APC7 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human APC7.The immunogen is located within amino acids 520 – 570 of APC7.
Host Species: Rabbit
Purification: APC7 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 115103-85-0
Product: MK-571 (sodium salt)
Buffer: APC7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: APC7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APC7 Antibody: APC7, APC7, Anaphase-promoting complex subunit 7, Cyclosome subunit 7
Accession NO.: NP_057322
Protein Ino: 212549736
Official Symbol: ANAPC7
Geneid: 51434
Background: APC7 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators.
PubMed ID:http://aac.asm.org/content/53/2/811.abstract
