Product Name: APC5 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: APC5 antibody can be used for detection of APC5 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.The immunogen is located within amino acids 480 – 530 of APC5.
Host Species: Rabbit
Purification: APC5 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 191729-45-0
Product: Tenacissoside H
Buffer: APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: APC5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APC5 Antibody: APC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5
Accession NO.: Q9UJX4
Protein Ino: 37537861
Official Symbol: ANAPC5
Geneid: 51433
Background: APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
PubMed ID:http://aac.asm.org/content/53/2/794.abstract
