Product Name: APC10 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: APC10 antibody can be used for detection of APC10 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APC10 antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10.The immunogen is located within amino acids 60 – 110 of APC10.
Host Species: Rabbit
Purification: APC10 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 478-01-3
Product: Nobiletin
Buffer: APC10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: APC10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APC10 Antibody: DOC1, APC10, Anaphase-promoting complex subunit 10, Cyclosome subunit 10
Accession NO.: EAX05052
Protein Ino: 119625457
Official Symbol: ANAPC10
Geneid: 10393
Background: APC10 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.
PubMed ID:http://aac.asm.org/content/53/2/832.abstract
