Product Name: AP2M1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1.The immunogen is located within amino acids 360 – 410 of AP2M1.
Host Species: Chicken
Purification: AP2M1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 479-92-5
Product: Propyphenazone
Buffer: AP2M1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: AP2M1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AP2M1 Antibody: mu2, AP50, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain
Accession NO.: NP_004059
Protein Ino: 14917109
Official Symbol: AP2M1
Geneid: 1173
Background: AP2M1 Antibody: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
PubMed ID:http://aac.asm.org/content/53/1/180.abstract
