Product Name: ALK (phospho Tyr1507) Antibody
Species Reactivity: Human, Mouse
Tested Applications: WB
Applications: Western Blot: 1:500~1:1000
User Note:
Predicted Molecular Weight: 176 kDa
Immunogen: ALK (phospho Tyr1507) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 1507 (P-T-Y (p)-G-S) derived from Human ALK.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 110652-62-5
Product: Peptide M
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: kinase ALK, Anaplastic lymphoma kinase, CD246, ALK tyrosine kinase receptor precursor,
Accession NO.: NP_004295.2
Protein Ino: 110347475
Official Symbol: Alk
Geneid: 238
Background: Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
PubMed ID:http://aac.asm.org/content/52/9/3276.abstract
