Product Name: ALF Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 50 kDa, 52 kDa, 132 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 94-09-7
Product: Benzocaine
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store ALF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ALF, ALF
Accession NO.: NP_751946
Protein Ino: 26787966
Official Symbol: GTF2A1L
Geneid: 11036
Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
PubMed ID:http://aac.asm.org/content/52/9/3369.abstract