Product Name: AIF Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, IF, WB
Applications: AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 67 kDa Observed: 67 kDa
Immunogen: AIF antibody was raised against a peptide corresponding to amino acids near the amino terminus of mature human AIF.The immunogen is located within amino acids 90 – 140 of AIF.
Host Species: Rabbit
Purification: AIF Antibody is Ion exchange chromatography purified.
Physical State: Liquid
CAS NO.: 293754-55-9
Product: T0901317
Buffer: AIF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
Storage Conditions: AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
Accession NO.: O95381
Protein Ino: 50400606
Official Symbol: CNKSR1
Geneid: 10256
Background: AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
PubMed ID:http://aac.asm.org/content/52/6/2053.abstract
