Product Name: ADAM10 Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, IF, WB
Applications: ADAM10 can be used for detection of ADAM10 by Western blot at 1 – 2 μg/mL. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 85 kDa
Immunogen: ADAM10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10.The immunogen is located within the last 50 amino acids of ADAM10.
Host Species: Rabbit
Purification: ADAM10 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1532533-69-9
Product: TGR-1202 (R-enantiomer)
Buffer: ADAM10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: ADAM10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ADAM10 Antibody: RAK, kuz, AD10, AD18, MADM, CD156c, HsT18717, KUZ, Disintegrin and metalloproteinase domain-containing protein 10, CDw156, ADAM 10
Accession NO.: NP_001101
Protein Ino: 4557251
Official Symbol: ADAM10
Geneid: 102
Background: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.
PubMed ID:http://aac.asm.org/content/52/3/1101.abstract
