Product Name: ACTH Antibody [AH26]
Species Reactivity: Human, Mouse, Rat
Tested Applications: Flow, IF, IHC
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A synthetic peptide corresponding to aa 1-24 of human ACTH was used as the immunogen for this ACTH antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 3681-99-0
Product: Puerarin
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin, LPH, MSH, NPP
Accession NO.:
Protein Ino:
Official Symbol: POMC
Geneid: 5443
Background: POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH (also called Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. Clone AH26 antibody is specific to Synacthen (aa 1-24 of ACTH) and does not react with CLIP (aa 17-39). ACTH is produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Specific antibody is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) that cause paraneoplastic syndromes by secreting ACTH.
PubMed ID:http://aac.asm.org/content/52/2/790.abstract
