Product Name: 4E-T Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5μg/ml Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: 4E-T antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of 4E-T.
Host Species: Goat
Purification: 4E-T antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 817194-38-0
Product: GSK180736A
Buffer: 4E-T antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter
Accession NO.: NP_062817.1
Protein Ino: 9789865
Official Symbol: EIF4ENIF1
Geneid: 56478
Background:
PubMed ID:http://aac.asm.org/content/51/11/3983.abstract
