Product Name: 14-3-3 sigma Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.03-0.1μg/ml. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: 14-3-3 sigma antibody was raised against a 14 amino acid synthetic peptide near the internal region of 14-3-3 sigma.
Host Species: Goat
Purification: 14-3-3 sigma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 516-54-1
Product: Allopregnanolone
Buffer: 14-3-3 sigma antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: SFN, stratifin, HGNC:10773, 14-3-3 sigma, HME1
Accession NO.: NP_006133.1
Protein Ino: 5454052
Official Symbol: SFN
Geneid: 2810
Background:
PubMed ID:http://aac.asm.org/content/51/10/3634.abstract
